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animal free blocking reagent  (Vector Laboratories)


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    Structured Review

    Vector Laboratories animal free blocking reagent
    Animal Free Blocking Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/animal+free+blocking+reagent/Animal-Free+Blocker+(5x)/pmc13134631-143-11-14
    Average 95 stars, based on 206 article reviews
    animal free blocking reagent - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Physical, chemical, and structural properties of human gastric organoid-derived mucus
    Article Snippet: Sections were stained with an Alcian blue-acetic acid solution (pH 2.5, Sigma) for 30 minutes and counterstained with nuclear fast red for 5 min. For detection of mucins MUC5AC and MUC6 by immunofluorescence, slides were then treated to heat-induced epitope retrieval using Vector Antigen Unmasking Solution in a rice cooker ( ). .. Sections were blocked for 30 min with animal-free blocking reagent (Vector Laboratories) and incubated with primary antibodies to E-cadherin (Biolegend Cat. 324102), MUC5AC (Santa Cruz Biotech Cat. 398985), and MUC6 (Invitrogen Cat. PA5-50557) overnight at 4°C. .. Samples were washed with DPBS and secondary antibodies (goat anti-mouse IgG2a AlexaFluor 488, Cat. 1080-32; goat anti-mouse IgG1 AlexaFluor 488, Cat. 1070-30; and goat anti-rabbit IgG1 AlexaFluor 555, Cat. 4050-32, respectively, all from Southern Biotechnology) were added for 2 h at room temperature with gentle agitation.

    Article Title: RAGE Is Essential for Subretinal Fibrosis in Laser-Induced Choroidal Neovascularization: Therapeutic Implications
    Article Snippet: .. After permeabilization, the sections were blocked with an animal-free blocking reagent (#SP-5035-100; Vector Laboratories) for 30 minutes. .. The sections were then incubated overnight at 4°C with primary antibodies against αSMA (1:100 dilution; Sigma-Aldrich, St. Louis, MO, USA), collagen I (1:100 dilution; Cell Signaling Technology), CD206 (1:1000 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), pSmad2 (1:100 dilution, #3108; Cell Signaling Technology), and RAGE (1:75 dilution, #sc-365154; Santa Cruz Biotechnology) in the blocking buffer.

    Article Title: Stress-induced CXCL13 regulates pancreatic exocrine homeostasis, age-related chronic inflammation, and cancer progression
    Article Snippet: .. The immunofluorescence protocol was modified to detect CXCL13, in which an animal-free blocking reagent (Vector) was used as the blocking solution, followed by incubation with the primary antibody diluted in the animal-free blocking solution at 4°C overnight. ..

    Incubation:

    Article Title: Physical, chemical, and structural properties of human gastric organoid-derived mucus
    Article Snippet: Sections were stained with an Alcian blue-acetic acid solution (pH 2.5, Sigma) for 30 minutes and counterstained with nuclear fast red for 5 min. For detection of mucins MUC5AC and MUC6 by immunofluorescence, slides were then treated to heat-induced epitope retrieval using Vector Antigen Unmasking Solution in a rice cooker ( ). .. Sections were blocked for 30 min with animal-free blocking reagent (Vector Laboratories) and incubated with primary antibodies to E-cadherin (Biolegend Cat. 324102), MUC5AC (Santa Cruz Biotech Cat. 398985), and MUC6 (Invitrogen Cat. PA5-50557) overnight at 4°C. .. Samples were washed with DPBS and secondary antibodies (goat anti-mouse IgG2a AlexaFluor 488, Cat. 1080-32; goat anti-mouse IgG1 AlexaFluor 488, Cat. 1070-30; and goat anti-rabbit IgG1 AlexaFluor 555, Cat. 4050-32, respectively, all from Southern Biotechnology) were added for 2 h at room temperature with gentle agitation.

    Article Title: Stress-induced CXCL13 regulates pancreatic exocrine homeostasis, age-related chronic inflammation, and cancer progression
    Article Snippet: .. The immunofluorescence protocol was modified to detect CXCL13, in which an animal-free blocking reagent (Vector) was used as the blocking solution, followed by incubation with the primary antibody diluted in the animal-free blocking solution at 4°C overnight. ..

    Immunofluorescence:

    Article Title: Stress-induced CXCL13 regulates pancreatic exocrine homeostasis, age-related chronic inflammation, and cancer progression
    Article Snippet: .. The immunofluorescence protocol was modified to detect CXCL13, in which an animal-free blocking reagent (Vector) was used as the blocking solution, followed by incubation with the primary antibody diluted in the animal-free blocking solution at 4°C overnight. ..

    Modification:

    Article Title: Stress-induced CXCL13 regulates pancreatic exocrine homeostasis, age-related chronic inflammation, and cancer progression
    Article Snippet: .. The immunofluorescence protocol was modified to detect CXCL13, in which an animal-free blocking reagent (Vector) was used as the blocking solution, followed by incubation with the primary antibody diluted in the animal-free blocking solution at 4°C overnight. ..



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